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Opis: Gene Biotechnology - Helen H. Zhang, Peter B. Kaufman, Michael J. Welsh

Covering state-of-the-art technologies and a broad range of practical applications, the Third Edition of Gene Biotechnology presents tools that researchers and students need to understand and apply today's biotechnology techniques. Many of the currently available books in molecular biology contain only protocol recipes, failing to explain the principles and concepts behind the methods outlined or to inform the reader of possible pitfalls in the methods described. Filling these gaps, this book: Discusses a wide variety of approaches, from very basic methods to the latest, most sophisticated technologies Contains clearly detailed, step-by-step protocols with helpful troubleshooting tips Addresses the needs of researchers in academic and commercial environments Guides graduate students in designing, implementing, and evaluating experimental projects. Each chapter covers the principles underlying methods and techniques, and includes step-by-step descriptions of each protocol, notes, tips, and a troubleshooting guide. The book includes sections on how to write a research paper for publication in English-language journals, how to protect research discoveries and inventions via patents, and practical methods of bio-calculation. Written by a team of internationally recognized scientists, Gene Biotechnology presents protocols as well as clear and simple explanations of the key principles and concepts behind the methods. It is a single, logically organized source for the most important new methodologies. This unique resource provides the tools to help ensure success in contemporary molecular and cellular biology research.Strategies for Novel Research Projects and Research Grant Funding Rapid Isolation of Specific cDNA or Gene by PCR Construction and Screening of Subtracted and Complete Expression cDNA Libraries Subcloning of Genes or DNA Fragments Non-isotopic and Isotopic DNA or RNA Sequencing Information Superhighway and Computer Databases of Nucleic Acids & Proteins Characterization of DNA or Genes by Southern Blot Hybridization Gene Over-expression by Sense RNA in Mammalian System Gene Under-expression by Antisense DNA and RNA Strategies Analysis of Gene Expression at the Functional Genomic Level Using Northern blotting and/or PCR Analysis of Gene Expression at the Proteomic Level via Western Blotting Analysis of Protein Expression Using Immuno-fluorescence Localization Analysis of Cellular DNA or Abundance mRNA by Radioactivity In Situ Hybridization (RISH) Localization of DNA or Abundance mRNA by Fluorescence In Situ Hybridization (FISH) In Situ PCR Hybridization of Low Copy Gene and In Situ RT-PCR Detection of Low Abundance mRNA Isolation and Characterization of Genes from Genomic DNA Libraries Mouse Stem Cells as a Model Mammalian Cell line for Gene Expression New Strategies for Gene Knockout Large-scale Expression and Purification of Recombinant Proteins Quantitative Analysis of Functional Genome by Real-Time RT -PCR High-throughput Analysis of Gene Expression by DNA Microarrays (Gene Chips) Construction and Screening of Human Antibody Libraries Using Phage Display Technology Down-regulation of Gene Expression in Mammalian Systems via SiRNA Technology New Techniques for Identification of Protein Binding and Proteomic Interactions


Szczegóły: Gene Biotechnology - Helen H. Zhang, Peter B. Kaufman, Michael J. Welsh

Tytuł: Gene Biotechnology
Autor: Helen H. Zhang, Peter B. Kaufman, Michael J. Welsh
Producent: CRC Press Inc.
ISBN: 9781439848302
Rok produkcji: 2011
Ilość stron: 575
Oprawa: Twarda
Waga: 0.95 kg


Recenzje: Gene Biotechnology - Helen H. Zhang, Peter B. Kaufman, Michael J. Welsh
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Covering state-of-the-art technologies and a broad range of practical applications, the Third Edition of Gene Biotechnology presents tools that researchers and students need to understand and apply today's biotechnology techniques. Many of the currently available books in molecular biology contain only protocol recipes, failing to explain the principles and concepts behind the methods outlined or to inform the reader of possible pitfalls in the methods described. Filling these gaps, this book: Discusses a wide variety of approaches, from very basic methods to the latest, most sophisticated technologies Contains clearly detailed, step-by-step protocols with helpful troubleshooting tips Addresses the needs of researchers in academic and commercial environments Guides graduate students in designing, implementing, and evaluating experimental projects. Each chapter covers the principles underlying methods and techniques, and includes step-by-step descriptions of each protocol, notes, tips, and a troubleshooting guide. The book includes sections on how to write a research paper for publication in English-language journals, how to protect research discoveries and inventions via patents, and practical methods of bio-calculation. Written by a team of internationally recognized scientists, Gene Biotechnology presents protocols as well as clear and simple explanations of the key principles and concepts behind the methods. It is a single, logically organized source for the most important new methodologies. This unique resource provides the tools to help ensure success in contemporary molecular and cellular biology research.Strategies for Novel Research Projects and Research Grant Funding Rapid Isolation of Specific cDNA or Gene by PCR Construction and Screening of Subtracted and Complete Expression cDNA Libraries Subcloning of Genes or DNA Fragments Non-isotopic and Isotopic DNA or RNA Sequencing Information Superhighway and Computer Databases of Nucleic Acids & Proteins Characterization of DNA or Genes by Southern Blot Hybridization Gene Over-expression by Sense RNA in Mammalian System Gene Under-expression by Antisense DNA and RNA Strategies Analysis of Gene Expression at the Functional Genomic Level Using Northern blotting and/or PCR Analysis of Gene Expression at the Proteomic Level via Western Blotting Analysis of Protein Expression Using Immuno-fluorescence Localization Analysis of Cellular DNA or Abundance mRNA by Radioactivity In Situ Hybridization (RISH) Localization of DNA or Abundance mRNA by Fluorescence In Situ Hybridization (FISH) In Situ PCR Hybridization of Low Copy Gene and In Situ RT-PCR Detection of Low Abundance mRNA Isolation and Characterization of Genes from Genomic DNA Libraries Mouse Stem Cells as a Model Mammalian Cell line for Gene Expression New Strategies for Gene Knockout Large-scale Expression and Purification of Recombinant Proteins Quantitative Analysis of Functional Genome by Real-Time RT -PCR High-throughput Analysis of Gene Expression by DNA Microarrays (Gene Chips) Construction and Screening of Human Antibody Libraries Using Phage Display Technology Down-regulation of Gene Expression in Mammalian Systems via SiRNA Technology New Techniques for Identification of Protein Binding and Proteomic Interactions

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Cena 523,95 PLN
Nasza cena 498,80 PLN
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Wysyłka: Niedostępna
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Szczegóły: Gene Biotechnology - Helen H. Zhang, Peter B. Kaufman, Michael J. Welsh

Tytuł: Gene Biotechnology
Autor: Helen H. Zhang, Peter B. Kaufman, Michael J. Welsh
Producent: CRC Press Inc.
ISBN: 9781439848302
Rok produkcji: 2011
Ilość stron: 575
Oprawa: Twarda
Waga: 0.95 kg


Recenzje: Gene Biotechnology - Helen H. Zhang, Peter B. Kaufman, Michael J. Welsh

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