Cascade Biocatalysis

  • Producent: VCH
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Opis: Cascade Biocatalysis

This ready reference presents environmentally friendly and stereoselective methods of modern biocatalysis. The experienced and renowned team of editors have gathered top international authors for this book. They cover such emerging topics as chemoenzymatic methods and multistep enzymatic reactions, while showing how these novel methods and concepts can be used for practical applications. Multidisciplinary topics, including directed evolution, dynamic kinetic resolution, and continuous-flow methodology are also discussed.§§From the contents:§Directed Evolution of Ligninolytic Oxidoreductases: from Functional Expression to Stabilization and Beyond§New Trends in the In Situ Enzymatic Recycling of NAD(P)(H) Cofactors§Monooxygenase-Catalyzed Redox Cascade Biotransformations§Biocatalytic Redox Cascades Involving w-Transaminases§Multi-Enzyme Systems and Cascade Reactions Involving Cytochrome P450 Monooxygenases§Chemo-Enzymatic Cascade Reactions for the Synthesis of Glycoconjugates§Synergies of Chemistry and Biochemistry for the Production of Beta-Amino Acids§Racemizable Acyl Donors for Enzymatic Dynamic Kinetic Resolution§Stereoselective Hydrolase-Catalyzed Processes in Continuous-Flow Mode§Perspectives on Multienzyme Process Technology§Nitrile Converting Enzymes Involved in Natural and Synthetic Cascade Reactions§Mining Genomes for Nitrilases§Key-Study on the Kinetic Aspects of the In-Situ NHase/AMase Cascade System of M. imperiale Resting Cells for Nitrile Bioconversion§Enzymatic Stereoselective Synthesis of Beta-Amino Acids§New Applications of Transketolase: Cascade Reactions for Assay Development§Aldolases as Catalyst for the Synthesis of Carbohydrates and Analogs§Enzymatic Generation of Sialoconjugate Diversity§Methyltransferases in Biocatalysis§Chemoenzymatic Multistep One-Pot ProcessesPreface§§DIRECTED EVOLUTION OF LIGNINOLYTIC OXIDOREDUCTASES: FROM FUNCTIONAL EXPRESSION TO STABILIZATION AND BEYOND§Introduction§Directed Molecular Evolution§The Ligninolytic Enzymatic Consortium§Directed Evolution of Laccases§Directed Evolution of Peroxidases and Peroxygenases§Saccharomyces cerevisiae Biomolecular Tool Box§Conclusions and Outlook§§NEW TRENDS IN THE IN SITU ENZYMATIC RECYCLING OF NAD(P)(H) COFACTORS§Introduction§Recent Advancements in the Enzymatic Methods for the Recycling of NAD(P)(H) Coenzymes and Novel Regeneration Systems§Conclusions§§MONOOXYGENASE-CATALYZED REDOX CASCADE BIOTRANSFORMATIONS§Introduction§Enzymatic Oxygenation§Effective Cofactor Recycling§In Vitro Multistep Biocatalysis§Combined In Vitro and In Vivo Multistep Biocatalysis§In Vivo Multistep Biocatalysis§Chemo-Enzymatic Cascade Reactions§Conclusions and Outlook§§BIOCATALYTIC REDOX CASCADES INVOLVING W-TRANSAMINASES§Introduction§General Features of w-Transaminases§Linear Cascade Reactions Involving w-Transaminases§Concluding Remarks§§MULTI-ENZYME SYSTEMS AND CASCADE REACTIONS INVOLVING CYTOCHROME P450 MONOOXYGENASES§Introduction§Physiological Cascade Reactions Involving P450s§Artificial Cascade Reactions Involving P450s§Conclusions and Outlook§§CHEMO-ENZYMATIC CASCADE REACTIOS FOR THE SYNTHESIS OF GLYCOCONJUGATES§Introduction§Sequential Syntheses§One-Pot Syntheses§Convergent Syntheses§Conclusion§§SYNERGIES OF CHEMISTRY AND BIOCHEMISTRY FOR THE PRODUCTION OF BETA-AMINO ACIDS§Introduction§Dihydropyrimidinase§N-Carbomoyl-Beta-Alanine Amidohydrolase§Bienzymatic System for Beta-Amino Acid Production§Conclusions and Outlook§§RACEMIZABLE ACYL DONORS FOR ENZYMATIC DYNAMIC KINETIC RESOLUTION§Introduction§The Tools§Applications of DKR to Acyl Compounds§Conclusions§§STEREOSELECTIVE HYDROLASE-CATALYZED PROCESSES IN CONTINUOUS-FLOW MODE§Introduction§Enzyme-Catalyzed Stereoselective Reactions in Continuous-Flow Systems§Outlook and Perspectives§§PERSPECTIVES ON MULTIENZYME PROCESS TECHNOLOGY§Introduction§Multienzyme System Classification§Biocatalyst Options§Reactor Options§Process Development§Process Modeling§Future§Concluding Remarks§§NITRILE CONVERTING ENZYMES INVOLVED IN NATURAL AND SYNTHETIC CASCADE REACTIONS§Introduction§Natural Cascades§Artificial Cascades§Conclusions and Future Use of These Enzymes§§MINING GENOMES FOR NITRILASES§Strategies in Nitrilase Search§Diversity of Nitrilase Sequences§Structure-Function Relationships§Enyzme Properties and Applications§Conclusions§§KEY-STUDY ON THE KINETIC ASPECTS OF THE IN SITU NHASE/AMASE CASCADE SYSTEM OF M. imperiale RESTING CELLS FOR NITRILE BIOCONVERSION§Introduction§The Temperature Effect on the NHase-Amidase Bi-Enzymatic Cascade System§Effect of Nitrile Concentration on NHase Activity and Stability§Effect of Nitrile on the AMase Activity and Stability§Concluding Remarks§§ENZYMATIC STEREOSELECTIVE SYNTHESIS OF BETA-AMINO ACIDS§Introduction§Preparation of Beta-Amino Acids§Nitrile Hydrolysis Enzymes§Conclusion§§NEW APPLICATIONS OF TRANSKETOLASE: CASCADE REACTIONS FOR ASSAY DEVELOPMENT§Introduction§Cascade Reactions for Assaying Transketolase Activity In Vitro Selection§Conclusion§§ALDOLASES AS CATALYST FOR THE SYNTHESIS OF CARBOHYDRATES AND ANALOGS§Introduction§Iminocyclitol and Aminocyclitol Synthesis§Carbohydrates and Other Polyhydroxylated Compounds§Conclusions§§ENZYMATIC GENERATION OF SIALCONJUGATE DIVERSITY§Introduction§A Generic Strategy for the Synthesis of Sialconjugate Libraries§Cascade Synthesis of Neo-Sialconjugates§Conclusions§§METHYLTRANSFERASES IN BIOCATALYSIS§Introduction§SAM-Dependent Methyltransferases§Conclusion and Outlook§§CHEMOENZYMATIC MULTISTEP ONE-POT PROCESSES§Introduction: Why Chemoenzymatic Cascades and Why One-Pot Processes?§Concepts of Chemoenzymatic Processes§Combination of Substrate Isomerization and their Derivatization with Chemo- and Biocatalysts Resul


Szczegóły: Cascade Biocatalysis

Tytuł: Cascade Biocatalysis
Producent: VCH
ISBN: 9783527335220
Rok produkcji: 2014
Ilość stron: 488
Oprawa: Twarda
Waga: 1.19 kg


Recenzje: Cascade Biocatalysis

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Cascade Biocatalysis

  • Producent: VCH
  • Oprawa: Twarda

This ready reference presents environmentally friendly and stereoselective methods of modern biocatalysis. The experienced and renowned team of editors have gathered top international authors for this book. They cover such emerging topics as chemoenzymatic methods and multistep enzymatic reactions, while showing how these novel methods and concepts can be used for practical applications. Multidisciplinary topics, including directed evolution, dynamic kinetic resolution, and continuous-flow methodology are also discussed.§§From the contents:§Directed Evolution of Ligninolytic Oxidoreductases: from Functional Expression to Stabilization and Beyond§New Trends in the In Situ Enzymatic Recycling of NAD(P)(H) Cofactors§Monooxygenase-Catalyzed Redox Cascade Biotransformations§Biocatalytic Redox Cascades Involving w-Transaminases§Multi-Enzyme Systems and Cascade Reactions Involving Cytochrome P450 Monooxygenases§Chemo-Enzymatic Cascade Reactions for the Synthesis of Glycoconjugates§Synergies of Chemistry and Biochemistry for the Production of Beta-Amino Acids§Racemizable Acyl Donors for Enzymatic Dynamic Kinetic Resolution§Stereoselective Hydrolase-Catalyzed Processes in Continuous-Flow Mode§Perspectives on Multienzyme Process Technology§Nitrile Converting Enzymes Involved in Natural and Synthetic Cascade Reactions§Mining Genomes for Nitrilases§Key-Study on the Kinetic Aspects of the In-Situ NHase/AMase Cascade System of M. imperiale Resting Cells for Nitrile Bioconversion§Enzymatic Stereoselective Synthesis of Beta-Amino Acids§New Applications of Transketolase: Cascade Reactions for Assay Development§Aldolases as Catalyst for the Synthesis of Carbohydrates and Analogs§Enzymatic Generation of Sialoconjugate Diversity§Methyltransferases in Biocatalysis§Chemoenzymatic Multistep One-Pot ProcessesPreface§§DIRECTED EVOLUTION OF LIGNINOLYTIC OXIDOREDUCTASES: FROM FUNCTIONAL EXPRESSION TO STABILIZATION AND BEYOND§Introduction§Directed Molecular Evolution§The Ligninolytic Enzymatic Consortium§Directed Evolution of Laccases§Directed Evolution of Peroxidases and Peroxygenases§Saccharomyces cerevisiae Biomolecular Tool Box§Conclusions and Outlook§§NEW TRENDS IN THE IN SITU ENZYMATIC RECYCLING OF NAD(P)(H) COFACTORS§Introduction§Recent Advancements in the Enzymatic Methods for the Recycling of NAD(P)(H) Coenzymes and Novel Regeneration Systems§Conclusions§§MONOOXYGENASE-CATALYZED REDOX CASCADE BIOTRANSFORMATIONS§Introduction§Enzymatic Oxygenation§Effective Cofactor Recycling§In Vitro Multistep Biocatalysis§Combined In Vitro and In Vivo Multistep Biocatalysis§In Vivo Multistep Biocatalysis§Chemo-Enzymatic Cascade Reactions§Conclusions and Outlook§§BIOCATALYTIC REDOX CASCADES INVOLVING W-TRANSAMINASES§Introduction§General Features of w-Transaminases§Linear Cascade Reactions Involving w-Transaminases§Concluding Remarks§§MULTI-ENZYME SYSTEMS AND CASCADE REACTIONS INVOLVING CYTOCHROME P450 MONOOXYGENASES§Introduction§Physiological Cascade Reactions Involving P450s§Artificial Cascade Reactions Involving P450s§Conclusions and Outlook§§CHEMO-ENZYMATIC CASCADE REACTIOS FOR THE SYNTHESIS OF GLYCOCONJUGATES§Introduction§Sequential Syntheses§One-Pot Syntheses§Convergent Syntheses§Conclusion§§SYNERGIES OF CHEMISTRY AND BIOCHEMISTRY FOR THE PRODUCTION OF BETA-AMINO ACIDS§Introduction§Dihydropyrimidinase§N-Carbomoyl-Beta-Alanine Amidohydrolase§Bienzymatic System for Beta-Amino Acid Production§Conclusions and Outlook§§RACEMIZABLE ACYL DONORS FOR ENZYMATIC DYNAMIC KINETIC RESOLUTION§Introduction§The Tools§Applications of DKR to Acyl Compounds§Conclusions§§STEREOSELECTIVE HYDROLASE-CATALYZED PROCESSES IN CONTINUOUS-FLOW MODE§Introduction§Enzyme-Catalyzed Stereoselective Reactions in Continuous-Flow Systems§Outlook and Perspectives§§PERSPECTIVES ON MULTIENZYME PROCESS TECHNOLOGY§Introduction§Multienzyme System Classification§Biocatalyst Options§Reactor Options§Process Development§Process Modeling§Future§Concluding Remarks§§NITRILE CONVERTING ENZYMES INVOLVED IN NATURAL AND SYNTHETIC CASCADE REACTIONS§Introduction§Natural Cascades§Artificial Cascades§Conclusions and Future Use of These Enzymes§§MINING GENOMES FOR NITRILASES§Strategies in Nitrilase Search§Diversity of Nitrilase Sequences§Structure-Function Relationships§Enyzme Properties and Applications§Conclusions§§KEY-STUDY ON THE KINETIC ASPECTS OF THE IN SITU NHASE/AMASE CASCADE SYSTEM OF M. imperiale RESTING CELLS FOR NITRILE BIOCONVERSION§Introduction§The Temperature Effect on the NHase-Amidase Bi-Enzymatic Cascade System§Effect of Nitrile Concentration on NHase Activity and Stability§Effect of Nitrile on the AMase Activity and Stability§Concluding Remarks§§ENZYMATIC STEREOSELECTIVE SYNTHESIS OF BETA-AMINO ACIDS§Introduction§Preparation of Beta-Amino Acids§Nitrile Hydrolysis Enzymes§Conclusion§§NEW APPLICATIONS OF TRANSKETOLASE: CASCADE REACTIONS FOR ASSAY DEVELOPMENT§Introduction§Cascade Reactions for Assaying Transketolase Activity In Vitro Selection§Conclusion§§ALDOLASES AS CATALYST FOR THE SYNTHESIS OF CARBOHYDRATES AND ANALOGS§Introduction§Iminocyclitol and Aminocyclitol Synthesis§Carbohydrates and Other Polyhydroxylated Compounds§Conclusions§§ENZYMATIC GENERATION OF SIALCONJUGATE DIVERSITY§Introduction§A Generic Strategy for the Synthesis of Sialconjugate Libraries§Cascade Synthesis of Neo-Sialconjugates§Conclusions§§METHYLTRANSFERASES IN BIOCATALYSIS§Introduction§SAM-Dependent Methyltransferases§Conclusion and Outlook§§CHEMOENZYMATIC MULTISTEP ONE-POT PROCESSES§Introduction: Why Chemoenzymatic Cascades and Why One-Pot Processes?§Concepts of Chemoenzymatic Processes§Combination of Substrate Isomerization and their Derivatization with Chemo- and Biocatalysts Resul

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