Chemical Biology

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Opis: Chemical Biology

This volume seeks to enable the discovery of tools in chemical biology by providing readers with various techniques ranging from initial chemical genetic screening to target identification. To successfully highlight the essential components of the chemical biology tool discovery process, the book is organizes into four parts that focus on platforms for molecular discovery in in vitro cellular systems, in vivo chemical genetic screening protocols, and methods used to discover functional protein targets. Written in the highly successful Methods of Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Practical and informative, Chemical Biology: Methods and Protocols seeks to improve the success rate of the chemical biology field through the dissemination of detailed and experiential knowledge.Part I In Vitro Chemical Genetic Screening 1. Identification of Therapeutic Small Molecule Leads in Cultured Cells Using Multiplexed Pathway Reporter Read-outs Ozlem Kulak, Kiyoshi Yamaguchi, and Lawrence Lum 2. Applying the Logic of Genetic Interaction to Discover Small Molecules That Functionally Interact with Human Disease Alleles Ari D. Brettman, Pauline H. Tan, Khoa Tran, and Stanley Y. Shaw 3. Construction and Application of a Photo-cross-linked Chemical Array Yasumitsu Kondoh, Kaori Honda, and Hiroyuki Osada 4. High Content Screening for Modulators of Cardiac Differentiation in Human Pluripotent Stem Cells Sean Spiering, Herman Davidovics, Paul J. Bushway, Mark Mercola, and Erik Willems 5. Small Molecule High-Throughput Screening Utilizing Xenopus Egg Extract Matthew R. Broadus, P. Renee Yew, Stephen R. Hann, and Ethan Lee Part II In Vivo Chemical Genetic Screening 6. Fission Yeast-based High-throughput Screens for PKA Pathway Inhibitors and Activators Ana Santos de Medeiros, Grace Kwak, Jordan Vanderhooft, Sam Rivera, Rachel Gottlieb, and Charles S. Hoffman 7. A Method for High-throughput Analysis of Chronological Aging in Schizosaccharomyces pombe Jessica Stephan and Ann E. Ehrenhofer-Murray 8. Protocols for the Routine Screening of Drug Sensitivity in the Human Parasite Trichomonas vaginalis Manal J. Natto, Anthonius A. Eze, and Harry P. de Koning 9. Chemical Genetic Screens Using Arabidopsis thaliana Seedlings Grown on Solid Medium Thanh Theresa Dinh and Xuemei Chen 10. Small Molecule Screening Using Drosophila Models of Human Neurological Disorders Mickael Poidevin, Feiran Zhang, and Peng Jin 11. High-throughput Small Molecule Screening in Caenorhabditis elegans Sunitha Rangaraju, Gregory M. Solis, and Michael Petrascheck 12. Whole-organism Screening for Modulators of Fasting Metabolism Using Transgenic Zebrafish Philipp Gut and Didier Y.R. Stainier 13. High Content Screening for Modulators of Cardiovascular or Global Developmental Pathways in Zebrafish Charles H. Williams III and Charles C. Hong Part III Small Molecules in Chemical Biology 14. Extraction Methods of Natural Products from Traditional Chinese Medicines Jingyu Zhang, Jianying Han, Ayokunmi Oyeleye, Miaomiao Liu, Xueting Liu, and Lixin Zhang 15. Bioassay-guided Identification of Bioactive Molecules from Traditional Chinese Medicines Jianying Han, Jingyu Zhang, Wenni He, Pei Huang, Ayokunmi Oyeleye, Xueting Liu, and Lixin Zhang 16. NMR Screening in Fragment-Based Drug Design: A Practical Guide Hai-Young Kim and Daniel F. Wyss 17. Practical Strategies for Small Molecule Probe Development in Chemical Biology Jonathan E. Hempel and Charles C. Hong 18. Principal Component Analysis as a Tool for Library Design: A Case Study Investigating Natural Products, Brand-name Drugs, Natural Product-like Libraries, and Drug-like Libraries Todd A. Wenderski, Christopher F. Stratton, Renato A. Bauer, Felix Kopp, and Derek S. Tan 19. Small Molecule Library Screening by Docking with PyRx Sargis Dallakyan and Arthur J. Olson Part IV Small Molecule Target Identification 20. Fluorous Photoaffinity Labeling to Probe Protein-Small Molecule Interactions Weigang Huang and Qisheng Zhang 21. Identification of the Targets of Biologically Active Small Molecules Using Quantitative Proteomics Gloria Vendrell-Navarro, Andreas Brockmeyer, Herbert Waldmann, Petra Janning, and Slava Ziegler 22. Drug Affinity Responsive Target Stability (DARTS) for Small Molecule Target Identification Melody Y. Pai, Brett Lomenick, Heejun Hwang, Robert Schiestl, William McBride, Joseph A. Loo, and Jing Huang 23. Chemical Genomic Profiling via Barcode Sequencing to Predict Compound Mode of Action Jeff S. Piotrowski, Scott W. Simpkins, Sheena C. Li, Raamesh Deshpande, Sean J. McIlwain, Irene M. Ong, Chad L. Myers, Charlie Boone, and Raymond J. Andersen 24. Image-Based Prediction of Drug Target in Yeast Shinsuke Ohnuki, Hiroki Okada, and Yoshikazu Ohya


Szczegóły: Chemical Biology

Tytuł: Chemical Biology
Producent: Humana Press
ISBN: 9781493922680
Rok produkcji: 2015
Ilość stron: 333
Oprawa: Twarda
Waga: 0.9 kg


Recenzje: Chemical Biology

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Chemical Biology

This volume seeks to enable the discovery of tools in chemical biology by providing readers with various techniques ranging from initial chemical genetic screening to target identification. To successfully highlight the essential components of the chemical biology tool discovery process, the book is organizes into four parts that focus on platforms for molecular discovery in in vitro cellular systems, in vivo chemical genetic screening protocols, and methods used to discover functional protein targets. Written in the highly successful Methods of Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Practical and informative, Chemical Biology: Methods and Protocols seeks to improve the success rate of the chemical biology field through the dissemination of detailed and experiential knowledge.Part I In Vitro Chemical Genetic Screening 1. Identification of Therapeutic Small Molecule Leads in Cultured Cells Using Multiplexed Pathway Reporter Read-outs Ozlem Kulak, Kiyoshi Yamaguchi, and Lawrence Lum 2. Applying the Logic of Genetic Interaction to Discover Small Molecules That Functionally Interact with Human Disease Alleles Ari D. Brettman, Pauline H. Tan, Khoa Tran, and Stanley Y. Shaw 3. Construction and Application of a Photo-cross-linked Chemical Array Yasumitsu Kondoh, Kaori Honda, and Hiroyuki Osada 4. High Content Screening for Modulators of Cardiac Differentiation in Human Pluripotent Stem Cells Sean Spiering, Herman Davidovics, Paul J. Bushway, Mark Mercola, and Erik Willems 5. Small Molecule High-Throughput Screening Utilizing Xenopus Egg Extract Matthew R. Broadus, P. Renee Yew, Stephen R. Hann, and Ethan Lee Part II In Vivo Chemical Genetic Screening 6. Fission Yeast-based High-throughput Screens for PKA Pathway Inhibitors and Activators Ana Santos de Medeiros, Grace Kwak, Jordan Vanderhooft, Sam Rivera, Rachel Gottlieb, and Charles S. Hoffman 7. A Method for High-throughput Analysis of Chronological Aging in Schizosaccharomyces pombe Jessica Stephan and Ann E. Ehrenhofer-Murray 8. Protocols for the Routine Screening of Drug Sensitivity in the Human Parasite Trichomonas vaginalis Manal J. Natto, Anthonius A. Eze, and Harry P. de Koning 9. Chemical Genetic Screens Using Arabidopsis thaliana Seedlings Grown on Solid Medium Thanh Theresa Dinh and Xuemei Chen 10. Small Molecule Screening Using Drosophila Models of Human Neurological Disorders Mickael Poidevin, Feiran Zhang, and Peng Jin 11. High-throughput Small Molecule Screening in Caenorhabditis elegans Sunitha Rangaraju, Gregory M. Solis, and Michael Petrascheck 12. Whole-organism Screening for Modulators of Fasting Metabolism Using Transgenic Zebrafish Philipp Gut and Didier Y.R. Stainier 13. High Content Screening for Modulators of Cardiovascular or Global Developmental Pathways in Zebrafish Charles H. Williams III and Charles C. Hong Part III Small Molecules in Chemical Biology 14. Extraction Methods of Natural Products from Traditional Chinese Medicines Jingyu Zhang, Jianying Han, Ayokunmi Oyeleye, Miaomiao Liu, Xueting Liu, and Lixin Zhang 15. Bioassay-guided Identification of Bioactive Molecules from Traditional Chinese Medicines Jianying Han, Jingyu Zhang, Wenni He, Pei Huang, Ayokunmi Oyeleye, Xueting Liu, and Lixin Zhang 16. NMR Screening in Fragment-Based Drug Design: A Practical Guide Hai-Young Kim and Daniel F. Wyss 17. Practical Strategies for Small Molecule Probe Development in Chemical Biology Jonathan E. Hempel and Charles C. Hong 18. Principal Component Analysis as a Tool for Library Design: A Case Study Investigating Natural Products, Brand-name Drugs, Natural Product-like Libraries, and Drug-like Libraries Todd A. Wenderski, Christopher F. Stratton, Renato A. Bauer, Felix Kopp, and Derek S. Tan 19. Small Molecule Library Screening by Docking with PyRx Sargis Dallakyan and Arthur J. Olson Part IV Small Molecule Target Identification 20. Fluorous Photoaffinity Labeling to Probe Protein-Small Molecule Interactions Weigang Huang and Qisheng Zhang 21. Identification of the Targets of Biologically Active Small Molecules Using Quantitative Proteomics Gloria Vendrell-Navarro, Andreas Brockmeyer, Herbert Waldmann, Petra Janning, and Slava Ziegler 22. Drug Affinity Responsive Target Stability (DARTS) for Small Molecule Target Identification Melody Y. Pai, Brett Lomenick, Heejun Hwang, Robert Schiestl, William McBride, Joseph A. Loo, and Jing Huang 23. Chemical Genomic Profiling via Barcode Sequencing to Predict Compound Mode of Action Jeff S. Piotrowski, Scott W. Simpkins, Sheena C. Li, Raamesh Deshpande, Sean J. McIlwain, Irene M. Ong, Chad L. Myers, Charlie Boone, and Raymond J. Andersen 24. Image-Based Prediction of Drug Target in Yeast Shinsuke Ohnuki, Hiroki Okada, and Yoshikazu Ohya

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Cena 418,01 PLN
Nasza cena 390,83 PLN
Oszczędzasz 6%
Wysyłka: Niedostępna
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Szczegóły: Chemical Biology

Tytuł: Chemical Biology
Producent: Humana Press
ISBN: 9781493922680
Rok produkcji: 2015
Ilość stron: 333
Oprawa: Twarda
Waga: 0.9 kg


Recenzje: Chemical Biology

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