Synthesis and Vaccine Evaluation of the Tumor Associated Carbohydrate Antigen Rm2 from Prostate Canc

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Opis: Synthesis and Vaccine Evaluation of the Tumor Associated Carbohydrate Antigen Rm2 from Prostate Canc - Hong-Yang Chuang

This thesis focuses on the synthesis and vaccine evaluation of the prostate tumor- associated carbohydrate antigen RM2. The author first presents the use of the [1+2+3] one-pot sequential strategy to successfully synthesise the RM2 antigen and its analogues as single stereoisomers in every glycosylation step, producing good yields and stereoselectivity. He then introduces the conjugation of the synthetic RM2 antigen to the carrier protein CRM197 in an average number of 1-10 to create the prostate cancer vaccine candidate, which is combined with alpha-galactosylceramide C1, its analogue C34, or Alu. The results of the vaccination studies in mice are also described and indicate that the strongest anti-RM2 antigen titer is exhibited when one molecule of diphtheria toxin (DT) is conjugated with an average of 4.7 molecules of RM2 antigen (DT-RM4.7) and adjuvanted with the glycolipid C34. More importantly, the induced mouse antibodies mediate the effective complement-dependent cytotoxicity (CDC) against the prostate cancer cell line LNCap. The study presented in this thesis is the first ever to successfully synthesize this complex glycan molecule. Owing to the steric hindrance of the adjacent sialyl moiety, the introduction of two sialic acid units to the compact and rigid 3,4 di branched galactoside unit is very challenging and the ss-selective and efficient glycosylation of the galactosamine moiety at the 4-position of di branched galactose is also problematic.Chemical Synthesis of Proposed RM2 and Derivatives.- RM2 Antigen: Structural Characterization and Determination of KD,surf for Multivalent Carbohydrate-Protein Interaction.- RM2 Antigen: Synthesis of Glycoconjugates.- Synthesis of the Heptasaccharide RM2 Prostate Tumor Antigen: Chemical Synthesis of Heptasaccharide and Tetrasaccharide (Inner Core of the RM2 Antigen).


Szczegóły: Synthesis and Vaccine Evaluation of the Tumor Associated Carbohydrate Antigen Rm2 from Prostate Canc - Hong-Yang Chuang

Tytuł: Synthesis and Vaccine Evaluation of the Tumor Associated Carbohydrate Antigen Rm2 from Prostate Canc
Autor: Hong-Yang Chuang
Wydawnictwo: Springer Verlag
ISBN: 9783662468470
Rok wydania: 2015
Ilość stron: 108
Oprawa: Twarda
Waga: 0.36 kg


Recenzje: Synthesis and Vaccine Evaluation of the Tumor Associated Carbohydrate Antigen Rm2 from Prostate Canc - Hong-Yang Chuang

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Synthesis and Vaccine Evaluation of the Tumor Associated Carbohydrate Antigen Rm2 from Prostate Canc

This thesis focuses on the synthesis and vaccine evaluation of the prostate tumor- associated carbohydrate antigen RM2. The author first presents the use of the [1+2+3] one-pot sequential strategy to successfully synthesise the RM2 antigen and its analogues as single stereoisomers in every glycosylation step, producing good yields and stereoselectivity. He then introduces the conjugation of the synthetic RM2 antigen to the carrier protein CRM197 in an average number of 1-10 to create the prostate cancer vaccine candidate, which is combined with alpha-galactosylceramide C1, its analogue C34, or Alu. The results of the vaccination studies in mice are also described and indicate that the strongest anti-RM2 antigen titer is exhibited when one molecule of diphtheria toxin (DT) is conjugated with an average of 4.7 molecules of RM2 antigen (DT-RM4.7) and adjuvanted with the glycolipid C34. More importantly, the induced mouse antibodies mediate the effective complement-dependent cytotoxicity (CDC) against the prostate cancer cell line LNCap. The study presented in this thesis is the first ever to successfully synthesize this complex glycan molecule. Owing to the steric hindrance of the adjacent sialyl moiety, the introduction of two sialic acid units to the compact and rigid 3,4 di branched galactoside unit is very challenging and the ss-selective and efficient glycosylation of the galactosamine moiety at the 4-position of di branched galactose is also problematic.Chemical Synthesis of Proposed RM2 and Derivatives.- RM2 Antigen: Structural Characterization and Determination of KD,surf for Multivalent Carbohydrate-Protein Interaction.- RM2 Antigen: Synthesis of Glycoconjugates.- Synthesis of the Heptasaccharide RM2 Prostate Tumor Antigen: Chemical Synthesis of Heptasaccharide and Tetrasaccharide (Inner Core of the RM2 Antigen).

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Cena 369,00 PLN
Nasza cena 345,01 PLN
Oszczędzasz 6%
Wysyłka: Niedostępna
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Szczegóły: Synthesis and Vaccine Evaluation of the Tumor Associated Carbohydrate Antigen Rm2 from Prostate Canc - Hong-Yang Chuang

Tytuł: Synthesis and Vaccine Evaluation of the Tumor Associated Carbohydrate Antigen Rm2 from Prostate Canc
Autor: Hong-Yang Chuang
Wydawnictwo: Springer Verlag
ISBN: 9783662468470
Rok wydania: 2015
Ilość stron: 108
Oprawa: Twarda
Waga: 0.36 kg


Recenzje: Synthesis and Vaccine Evaluation of the Tumor Associated Carbohydrate Antigen Rm2 from Prostate Canc - Hong-Yang Chuang

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